Composite

Part:BBa_K3096002

Designed by: Famke Baeuerle, Patrick Mueller, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)

RBS-AraC-Term

This part consists of an RBS and the coding sequence for the araC protein as well as an already existing Terminator (BBa_B0014). AraC is a repressor protein that binds to the pBAD promoter inhibiting the transcription of downstream genes. AraC inhibits gene expression in the absence of arabinose by binding to the operator units present within the araC and pBAD promoter sequences by producing a DNA loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References


[edit]
Categories
Parameters
None